FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26339669
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG3700.018.91772538
CCCGTAT25900.012.70399951
CGGGAAT31050.010.4841611
CTCGCGT10250.09.5619511
GTCGCAT23200.09.50528051
CTCGTAT48300.09.4937221
TATGCCG21650.09.37574546
GTCCGGT34500.09.0299061
CTCCGCT85150.09.002681
CGTATTA10200.08.9211063
GTCCGCT50600.08.78548051
ACGTCTG62000.08.74943115
GTCCGTT41350.08.7191491
CGCCTAT10850.08.7105661
CCCGTCT70800.08.7014531
CGCTAGT10600.08.5857821
CTCAGAT131700.08.5050441
CCCGAAT44100.08.4135571
CCCGCAT43100.08.3651231
CGTCTGA65850.08.34457716
Produced by FastQC (version 0.11.5)