FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34119417
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA13700.012.0067843
CCCGTAT32400.011.3440181
CGGGTAT30550.09.7393571
CCCGATT33800.08.9064451
CTCGTAT57150.08.6975061
GTCGCAT29500.08.6621061
GTCCGTT51450.08.6405411
CTCCGAT82300.08.6341351
GTCGGTT63500.08.5995051
GTCCGGT46650.08.32902051
CCCGAAT60250.08.3080981
GTCCGCA60600.08.2023511
TTAACCG18800.08.1910067
GTCCGCT67900.08.1453531
GTCGGAT37950.08.1169621
CGGGAAT46300.08.0895691
CCCGTTT68600.08.0622381
CTCGCGT13900.08.0585661
CCCGCAT56800.07.8883151
CTCGAAT102500.07.78634451
Produced by FastQC (version 0.11.5)