FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26054729
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG307550.11803999189552115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG39150.041.1209346
TCGTATG48550.033.66442543
CGTATGC50600.031.81638744
TATCTCG51000.031.70433639
CGCCAAT57700.028.5105533
ACGCCAA60650.027.12479432
GTCACGC63300.025.99108129
CTCGTAT64100.025.17028442
GTATGCC68700.023.7904645
ATGCCGT71400.022.84149747
AGTCACG73850.022.37433228
TCACGCC78650.020.96126230
ACACGTC83050.020.52270313
ACGTCTG84500.020.37766515
CGTCTGA87200.019.74719816
ATATCTC84300.019.7201638
TCTCGTA83450.019.41790441
CCAATAT86500.019.26013835
TGCCGTC87900.018.6732948
GCCGTCT92600.017.8767949
Produced by FastQC (version 0.11.5)