FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb166.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb166.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25602670
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC1092210.42660003819914094TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC148100.059.19667444
CTCGTAT141500.058.84169842
TCGTATG145000.058.82108743
TCTCGTA141050.058.1609841
TATGCCG151050.057.9936646
ATGCCGT150550.057.6980947
TGCCGTC153450.056.28825848
GCCGTCT151150.055.70921749
ATCTCGT148150.055.58615540
CCGTCTT153950.055.08245550
CATCTCG153100.053.97193539
CGTCTTC157050.053.12609551
CGTCTGA179650.051.3937216
ACGTCTG186550.049.47239315
ACACGTC187750.049.36124413
GTATGCC185200.047.75372345
CACGTCT197550.046.85939814
CTTAGGC210550.043.65331333
GCACACG213950.043.51283611
CACACGT214650.043.22419412
Produced by FastQC (version 0.11.5)