FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb165.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb165.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33579818
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC6014461.7910936860944273TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC1641570.4888561337646321TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG885150.064.4600943
CGTATGC919950.064.27383444
CTCGTAT702150.064.23904442
TATGCCG921800.064.1826246
TCTCGTA692800.064.18146541
GATCTCG696200.063.8933539
ATGCCGT918350.063.79483447
TGCCGTC912550.063.6288748
CCGTCTT903400.063.2504150
ATCTCGT701300.063.2439640
GCCGTCT896450.063.19830349
CGTCTTC891500.063.0778451
CGTCTGA1036400.062.4639816
ACACGTC1051050.061.97164513
ACGTCTG1046500.061.96694615
CACGTCT1057050.061.50400514
GTATGCC968100.061.44581245
GTCGTAT206000.060.3970742
CACACGT1082700.060.20873312
GCACACG1085600.060.14138811
Produced by FastQC (version 0.11.5)