FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb164.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb164.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40660010
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC6196651.5240158573497646TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC754550.063.0567744
TCGTATG754650.063.02985843
CTCGTAT728900.062.8467442
TCTCGTA723550.062.7408341
AATCTCG717650.062.66174339
TATGCCG759400.062.64452746
ATGCCGT761850.062.35578547
TGCCGTC763400.062.11457448
GCCGTCT766200.061.7185749
CCGTCTT768150.061.64390650
ATCTCGT731150.061.6100740
CGTCTTC768950.061.4841951
CGTCTGA816750.060.38268316
ACACGTC835700.059.34235413
ACGTCTG833100.059.27129415
GTATGCC807650.058.9325445
CACGTCT846050.058.56675714
CACACGT880650.056.40906512
GCACACG885000.056.18321211
GACCAAT838650.055.543635
Produced by FastQC (version 0.11.5)