FastQCFastQC Report
Sat 9 Feb 2019
HTTKYBGX9_n01_mb163.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTKYBGX9_n01_mb163.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35248273
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC8633192.4492519108666686TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG972950.065.5966243
CGTATGC1001000.065.4925444
CTCGTAT977350.065.4592342
TCTCGTA972500.065.418841
TATGCCG1003850.065.36880546
CGATGTA1067000.065.3287134
TATCTCG990550.065.0924539
ACCGATG1074550.065.08781432
ATGCCGT995950.065.0615247
CACCGAT1076600.065.0333531
CCGATGT1074100.064.95048533
TGCCGTC993200.064.8082548
ATCTCGT991000.064.7520240
GCCGTCT972050.064.504349
CCGTCTT982000.064.4743150
CGTCTGA1101350.064.2295616
CGTCTTC968250.064.17181451
TCACCGA1092150.064.1326830
ACACGTC1109800.064.0512713
GTCACCG1093700.063.98021329
Produced by FastQC (version 0.11.5)