FastQCFastQC Report
Sat 16 Feb 2019
HTTGHBGX9_n02_ba155.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTGHBGX9_n02_ba155.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences412710196
Sequences flagged as poor quality0
Sequence length98
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26317350.637671427918878No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAG14888620.3607524152371559No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGGCTGGTGAGATGGCTCA9646570.23373713791165945No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAACAAGCGAGATAGGAA6553410.15878963164748175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT17484950.072.048271
AGCAGTG19235600.065.396582
GGTATCA20761550.060.3942348
GTGGTAT20790450.060.3551866
GTATCAA21193550.059.2135629
TGGTATC21633550.057.9432077
AGTGGTA21924800.057.3367845
GCAGTGG22651950.055.5348663
CAGTGGT23234750.054.10884
ATCAACG20570800.033.93589810-11
CAACGCA21335750.032.7327612-13
ACGCAGA21429300.032.63266814-15
AGTACAT21510250.032.2591920-21
AGAGTAC22300850.031.2378718-19
GCAGAGT22640450.030.83910816-17
TACATGG22465100.030.57682822-23
TATCAAC20925600.030.09277510-11
TCAACGC20908300.030.0415512-13
CGCAGAG21049950.029.90868616-17
CATGGGG16665650.029.6664824-25
Produced by FastQC (version 0.11.5)