FastQCFastQC Report
Sun 21 Nov 2021
HTTCNDSX2_l02_n02_EP_HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTCNDSX2_l02_n02_EP_HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116961449
Sequences flagged as poor quality0
Sequence length101
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2990755025.57043389570182No Hit
GAGGCTGAGCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8933560.7638038068423725No Hit
TCGATAATGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8515870.7280920399678017No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7916180.6768195903592131No Hit
GAGGCTGAGCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7226270.6178334880239044No Hit
TCGATAATGCGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5618410.4803642608771032No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5614810.480056467152694No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5493850.46971459801254684No Hit
TCGATAATGCGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5401110.4617854896787402No Hit
GAGGCTGAGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5043000.43116770894314077No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4676560.3998377277285612No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4336610.3707725953360923No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3971790.3395811212974969No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3968240.3392776024859268No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3839390.3282611520997829No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3671990.31394874391475774No Hit
TCGATAATGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3644520.3116001068010024No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3085520.26380658126080503No Hit
TCGATAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2518540.21533077963150063No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2311590.19763691539081393No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180640.1864409186654314No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1776530.15189021811793732No Hit
ACTGTCTAACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1474390.1260577748143322No Hit
ACATGTTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1372130.11731472307597694No Hit
AGCTTACTATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1342750.11480278429177122No Hit
GTGTGACGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1335580.11418976179065633No Hit
ACTGTCTAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1268070.1084177744754171No Hit
GTGTGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1186280.10142487205335494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)