FastQCFastQC Report
Sun 21 Nov 2021
HTTCNDSX2_l02_n02_EP_HTO1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTCNDSX2_l02_n02_EP_HTO1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122354829
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2060285216.83860961466425No Hit
GAGGCTGAGCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10389030.8490903125695186No Hit
TCGATAATGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9482340.774986984780143No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9370920.7658806829765583No Hit
GAGGCTGAGCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8395290.6861429228919114No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6495090.5308405114112824No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6399760.523049237394627No Hit
TCGATAATGCGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6240190.510007659771238No Hit
TCGATAATGCGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6054600.4948394803444987No Hit
GAGGCTGAGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5923940.4841607028031562No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5521320.4512547682118864No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4958010.40521571894804415No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4954750.40494928075131387No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4708640.3848348314883428No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4547570.3716706596026545No Hit
TCGATAATGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4274280.3493348023068219No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4228120.34556216820833446No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3740210.30568552386273207No Hit
TCGATAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2937400.2400722573851172No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690040.21985564623689677No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2491080.20359474328553065No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2053330.16781765107121355No Hit
ACTGTCTAACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1839920.15037575672636508No Hit
ACTGTCTAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1595250.13037899795520128No Hit
GTGTGACGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1586200.12963934590599607No Hit
AGCTTACTATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1577650.12894055861089063No Hit
ACATGTTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570930.1283913363157902No Hit
GTGTGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1356460.1108628086922503No Hit
GAGGCTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1347260.11011089721681519No Hit
GTGTGACGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1293420.10571058049535585No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)