FastQCFastQC Report
Sun 21 Nov 2021
HTTCNDSX2_l01_n02_EP_HTO2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTCNDSX2_l01_n02_EP_HTO2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122056955
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3622824129.6814229062162No Hit
GAGGCTGAGCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8575530.7025842976338382No Hit
TCGATAATGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190860.6710686826490142No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7587750.6216565045392128No Hit
GAGGCTGAGCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6905870.5657907818526196No Hit
TCGATAATGCGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5412990.4434806685124989No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5383200.4410400046437338No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5322180.43604069919653493No Hit
TCGATAATGCGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5199120.42595852075778884No Hit
GAGGCTGAGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4880620.3998641453901582No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4488170.36771112305726456No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4194650.34366333323652065No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3836630.31433112517021256No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3823660.3132685064935464No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3696150.3028217441603389No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3566010.2921595086490565No Hit
TCGATAATGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3490400.28596485960181456No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950000.24169044689014238No Hit
TCGATAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2389730.19578810564297627No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2224310.18223541624481784No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2086380.17093495409581536No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1713730.1404041252708623No Hit
ACTGTCTAACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1413750.11582707433591145No Hit
ACATGTTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1314690.10771119105830552No Hit
AGCTTACTATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1289640.10565887048386552No Hit
GTGTGACGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1279560.10483302651618664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)