FastQCFastQC Report
Sun 21 Nov 2021
HTTCNDSX2_l01_n02_EP_HTO1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTTCNDSX2_l01_n02_EP_HTO1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131837853
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2631701819.96165547386455No Hit
GAGGCTGAGCTAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10532500.798898022102954No Hit
TCGATAATGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9656290.732436836634468No Hit
GTGTGACGTATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9443040.7162616642429698No Hit
GAGGCTGAGCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8496430.6444605859896703No Hit
AGGACCATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6625200.5025263874708275No Hit
GTGTGACGTATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6450060.4892418871536083No Hit
TCGATAATGCGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6345170.48128590200873494No Hit
TCGATAATGCGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6172500.4681887530434829No Hit
GAGGCTGAGCTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5991700.45447493748248463No Hit
GTGTGACGTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5600440.4247975731218863No Hit
AGGACCATCCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5069640.38453599513638925No Hit
ACTGTCTAACGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5043530.38255553205952164No Hit
ACTGTCTAACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4776300.3622859361946678No Hit
AGGACCATCCAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4651780.35284100083152903No Hit
AGGACCATCCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4337390.3289942836068485No Hit
TCGATAATGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4330710.32848760059828946No Hit
ACTGTCTAACGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3821210.2898416435831976No Hit
TCGATAATGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2949560.2237263375337279No Hit
ACATGTTACCGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2761060.20942847119939068No Hit
ACATGTTACCGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2544180.1929779605861755No Hit
ACATGTTACCGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2094730.15888684109562978No Hit
ACTGTCTAACGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1865890.14152915551499462No Hit
ACTGTCTAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1605320.12176472564370416No Hit
GTGTGACGTATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1603730.12164412295154715No Hit
AGCTTACTATCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1601520.1214764927945239No Hit
ACATGTTACCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1595200.12099711605588721No Hit
GTGTGACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1371920.10406116064405266No Hit
GAGGCTGAGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1359460.1031160603017405No Hit
GTGTGACGTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1319220.10006382613042097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)