FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35038857
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29844478.517535260924749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA204900.057.001131
ATCGGAA210750.055.7988972
TCGGAAG216750.053.285683
ACACGTC175500.052.956913
CGGAAGA217700.052.1530534
CACACGT185050.051.1842912
CGTCTGA175700.050.88899216
ACGTCTG177500.050.78701815
GCACACG189450.050.34387211
CACGTCT187000.049.1400214
AGAGCAC246750.041.5819138
AGCACAC251200.039.54012710
GAACTCC227950.037.31273721
CTGAACT236450.037.2161219
TGAACTC258250.033.9915920
AACTCCA265200.032.01885622
CCAGTCA218750.029.42449826
CTCCAGT259700.029.35147924
TCTGAAC324250.027.91700718
CAGTCAC208950.027.35409227
Produced by FastQC (version 0.11.5)