FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_mb181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_mb181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28395487
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT30150.013.0024271
TCGACCG26000.011.5766331
ACGTATA18600.011.4759818
ACGTACG22700.011.41181453
GACGTAT19050.011.20493317
CGTATTA12900.010.58159353
CTCCGTT73450.010.2933251
TACGTAC26800.010.188642552
CTCGTTT75350.09.8944151
CGTTTCG25350.09.80251331
CTCCGAT72500.09.6074651
GTTCGAC33550.09.49308629
CGACCGT33650.09.36037132
CCCGTCT77600.09.3368931
CGCCTAT12000.09.3338861
CGGGAAT36550.09.2891961
CCCGAAT56750.09.2516491
TCCGCTA15650.09.1694992
CGTACGA28450.08.98208554
CCCGTTT66500.08.9478991
Produced by FastQC (version 0.11.5)