FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_mb180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_mb180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30014831
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG20450.013.52374153
CCCGTAT28850.011.88952351
CGTACGA23500.011.767982554
GACGTAT21950.010.84049917
TACGTAC26850.010.43063852
CTCGTAT48200.010.0937361
CTCCGAT74900.09.953641
TACGAAA29350.09.89986856
ACGTATA23850.09.83015918
CTACGTA29700.09.66533451
CCCGTTT67450.09.60005951
GTCCGTA28400.09.4897881
CGTATAG27800.09.44026419
GTCCGTT44000.09.3867111
GTCCGCT57650.09.2891561
GTCCGGT38450.09.285131
CGTTTCG22250.09.28045531
GACTACG26050.09.26845935
CTCGCGT11400.09.2108691
CCCGCAT49750.09.1460681
Produced by FastQC (version 0.11.5)