FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_mb179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_mb179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31547715
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT23100.011.05830117
CTCGTAT55300.010.8863091
CCCGTAT33100.010.6799871
GTCGCAT24650.010.6493151
ACGTATA23800.010.58601118
CGTATTA13900.010.5751453
GTCCGTT48950.010.1534351
GTCCGGT42550.010.0354671
CGTATCG28650.010.01536417
ACGTACG20450.09.41513553
GTCCGCT64200.08.995521
CGGGAAT39750.08.9813251
CGCTAGT10950.08.9499641
TCCGCTA18100.08.8946822
GTCGGTT53050.08.7749281
CCCGTCT85500.08.7604211
GTCCGCA55850.08.585681
TCGTATC41950.08.4263712
TCGCATT38000.08.2891512
GACTACG32650.08.14523835
Produced by FastQC (version 0.11.5)