FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_mb178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_mb178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33508700
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT30800.014.8874931
CGTATTA15250.012.6230053
TCGACCG24250.011.4014831
GTCCGTT49300.011.0048921
ACGTACG24250.010.24976153
GTCCGGT44250.010.20416451
CCGTATT38350.010.1304362
GTCGGTT60200.010.0589031
CGGGAAT42150.09.7990831
CGACCGT31000.09.2571532
CGTTTCG28150.08.95158631
CTCGTAT55950.08.8836051
CGTACGA28450.08.85929654
CGTATCG34000.08.850944517
CCCGTTT72850.08.8407541
GTCCGCT63400.08.7782691
GTCGCGA9759.094947E-128.6160381
TACGTAC29700.08.60472152
GTCGCAT28650.08.5521331
CCCGAAT54050.08.5482891
Produced by FastQC (version 0.11.5)