FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_mb177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_mb177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32339630
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG28400.012.32700853
ACGTATA20550.011.06793618
GACGTAT21250.010.86807817
CCCGTAT34400.010.7850781
TACGTAC34100.010.6771452
CGTATCG38400.010.205976517
CGTACGA35000.010.0021354
GTCCGGT44550.09.742041
TACGAAA37550.09.50954156
TCGACCG30350.09.4556831
CTACGTA37600.09.40362751
GTCCGTT52800.09.346761
CGTATTA11600.09.0513143
GTCGCAT26800.09.0113571
GTCCGAA38950.08.8961811
TGCTACG42550.08.63879149
CTCGTAT56800.08.6269171
GTACGAA40700.08.6014255
CCCGTCT86800.08.5888651
CCCGAAT62450.08.4068781
Produced by FastQC (version 0.11.5)