FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_mb176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_mb176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35156481
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTACG31250.013.77899353
CCCGTAT34100.012.2144711
CGTTTCG35000.012.19930131
CGTACGA38500.011.365684554
TACGTAC38700.011.30746352
CGTATTA13800.010.65195753
GTCCGGT48800.010.6150971
ACGTATA22100.010.45029918
CTACGTA42750.010.39980251
GACGTAT22950.010.36824217
GTCCGTT52900.010.3216941
TACGAAA41950.010.18086556
TCGACCG36550.010.14989731
TGCTACG47900.09.8662949
CTCGTAT61650.09.70834351
CGTATCG38700.09.58459717
GCTACGT49750.09.56982250
TTACCGG43700.09.53437563
CCCGTTT81000.09.4632691
GTACGAA45800.09.40135255
Produced by FastQC (version 0.11.5)