FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_ba154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_ba154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30767100
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC604140.1963590978675273TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA95800.058.0181961
ACGGCTA90950.056.95074532
CGGCTAC91100.056.3182233
CTCGTAT89150.056.0547842
TCGTATG92000.055.88158443
TCTCGTA86250.055.4616341
ATCGGAA101000.055.2016942
CGTATGC94850.054.31574244
ATGCCGT94100.053.78887647
CGTCTGA102050.052.394616
ACACGTC105800.050.73438613
TCGGAAG110800.050.3507463
TATGCCG101800.050.26627346
TCACGGC104250.050.1915930
GTCACGG105400.049.84319729
TGCCGTC101850.049.66280448
ACGTCTG108650.049.34068715
CCGTCTT101750.049.30094550
GCCGTCT102550.048.77948449
CGGAAGA116150.047.760344
Produced by FastQC (version 0.11.5)