FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_ba153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_ba153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31368130
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC4540031.4473384291636129TruSeq Adapter, Index 10 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG454800.14498792245505232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA663650.066.702241
ATCGGAA671150.066.218392
CGTCTGA664100.065.6964316
TCTCGTA541500.065.3005741
ACACGTC668350.065.2827513
CTCGTAT551600.065.2433642
TCGTATG556450.065.1833243
ACGTCTG670000.065.17013515
CGTATGC581650.065.02701644
ATGCCGT568750.064.9858847
TCGGAAG684300.064.8844153
TATCTCG560550.064.5886839
CGGAAGA687100.064.365314
TATGCCG590250.064.1009446
ATCTCGT558800.063.88481540
GCCGTCT539800.063.84488749
TGCCGTC566450.063.83048648
CCGTCTT550900.063.5819450
CACGTCT689950.063.2802614
GCACACG694100.062.8979911
Produced by FastQC (version 0.11.5)