FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_ba152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_ba152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32897506
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC6982642.122543879162147TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334450.10166424166014286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA912200.068.35591
ATCGGAA917900.068.01862
TCGGAAG930200.066.961063
CGGAAGA930150.066.441654
ACACGTC934950.064.8976113
CGTCTGA930900.064.8312716
ACGTCTG937500.064.57641615
TCGTATG907750.064.3770343
CGATCAG915050.064.3142633
GTCACGA919000.064.26359629
CGTATGC914400.064.2523144
CTCGTAT868400.064.1744242
TCTCGTA846750.064.0472341
ACGATCA921200.064.03422532
CACGATC920950.063.94860531
ATGCCGT914600.063.84194647
TCACGAT927900.063.62079630
GATCTCG820400.063.56403439
GCACACG956550.063.49193211
TGCCGTC917250.063.45022648
Produced by FastQC (version 0.11.5)