FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_GG013.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_GG013.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28815476
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC861610.29900946283170887TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT116850.057.38132542
TCTCGTA116550.056.74461441
TCGTATG121400.055.9850443
CGTATGC129300.055.94988644
ATGCCGT127500.055.84205247
AATCTCG124100.054.81274439
TATGCCG136050.053.81963746
TCGCGTA11700.053.2364741
TGCCGTC129700.052.89980348
ATCTCGT127000.052.32245340
GCCGTCT124700.051.87872349
CCGTCTT128550.051.33239450
CGTCTGA180200.049.92497616
CGTCTTC131450.047.8563751
ACGTCTG190250.047.47160715
ACACGTC192000.046.85569413
CGCGTAT13600.046.57398642
ATCGCGT13850.045.4767740
GTATGCC168050.044.09102245
CACGTCT206850.043.6111214
Produced by FastQC (version 0.11.5)