FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_GG012.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_GG012.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23594072
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC9245113.918403741414369TruSeq Adapter, Index 3 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCAGCTCGTATGC458340.19426066005054152TruSeq Adapter, Index 3 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATGTCGTATGC282290.1196444598456765TruSeq Adapter, Index 3 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270710.11473644735847208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1197350.065.92496543
ATGCCGT1215850.065.9040447
CTCGTAT1139600.065.8656242
CGTATGC1226850.065.8563144
TCTCGTA1086700.065.74203541
TATGCCG1232100.065.5226346
TGCCGTC1212000.065.49748
CCGTCTT1190350.065.4686950
GCCGTCT1186400.065.4673549
CGTCTGA1297950.065.3433216
CGTCTTC1180000.065.3131451
ATCTCGT1080250.065.27840
ACGTCTG1303500.065.1993315
ACACGTC1305400.065.1644313
CATCTCG1084200.065.02602439
GTATGCC1251950.064.6491645
ATCGGAA1358150.064.5477752
GATCGGA1356050.064.402011
CACACGT1321300.064.3553412
CACGTCT1323050.064.31248514
Produced by FastQC (version 0.11.5)