FastQCFastQC Report
Sat 16 Feb 2019
HTNW7BGX9_n01_GG011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTNW7BGX9_n01_GG011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26078267
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC8051503.087436753370153TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG534820.2050826460209185No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGAGCTCGTATGC353270.1354652899289665TruSeq Adapter, Index 1 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGCTCTCGTATGC352320.13510100191857075TruSeq Adapter, Index 1 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1133350.067.0352251
ATCGGAA1139300.066.794962
CTCGTAT1012100.066.1796242
TCGTATG1082200.066.1139743
CGTATGC1091200.066.0330444
TCGGAAG1154400.065.927323
TCTCGTA967650.065.9247141
ATGCCGT1085400.065.827147
ACGATCT924200.065.6585837
CGATCTC927400.065.5868438
CACGATC930050.065.5579836
TATGCCG1095550.065.4545346
CGTCTGA1132550.065.4190316
TGCCGTC1088050.065.33211548
CGGAAGA1159350.065.31984
GATCTCG933550.065.2639839
TCACGAT997350.065.258635
CCGTCTT1080950.065.2218150
ACGTCTG1138000.065.1887515
ACACGTC1138650.065.1839713
Produced by FastQC (version 0.11.5)