FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23278426
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17006367.305631403085415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA175350.016.1215552
TCGGAAG171550.016.0337773
CGGAAGA176500.015.7707834
GATCGGA177550.015.3947321
TCGTGTA88000.014.7679914-15
GAGCGTC206150.012.8385579
AGCGTCG107450.012.11641210-11
GTAGGGA91300.011.99688218-19
TAGGGAA93200.011.47188718-19
AGAGCGT230400.011.466118
CGTGTAT16500.011.08724114-15
AAGAGCG254350.011.0208977
GTGTAGG101600.010.66349516-17
TGTAGGG102850.010.16428416-17
CTCGGAT27500.09.6801111
TATTACG12550.08.89511652-53
CGGGGAT33600.08.7715711
AGGGAAA151850.08.3092720-21
GAAGAGC353400.08.2668456
CGAATAG12201.2896635E-98.1772543
Produced by FastQC (version 0.11.5)