FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27736970
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG367600.011.56958480-81
TAGGGGG142950.010.51622550-51
AAAAAGG492250.010.154078578-79
CGGGAAT60800.09.8483371
AGGGGGG541150.09.23722582-83
CGGGGAT86950.08.9086921
TAAAAAA848100.08.61719570-71
CGGGGTT93900.08.4011441
GGCGAAT45700.08.3189741
GCCGATT30450.08.2715041
AAGGGGG361250.08.10517282-83
CGGGAAC90050.08.0742771
GCGTCGT229850.08.04012494-95
CTCGTAT25450.08.029281
CGCGGAT37250.07.9097241
ATCGGAA531500.07.838879694-95
GATCGGA564700.07.802859394-95
CGTCGTG224700.07.7909894-95
CCGGGAT86250.07.7688351
TACCGTA21600.07.6971939
Produced by FastQC (version 0.11.5)