FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34233346
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG405350.010.02366180-81
CGCGTAA10153.4560799E-98.8954931
CGGGGAT117600.08.5666491
CGCGTAT7251.6826974E-58.52094651
AGGGGGG607850.08.313580582-83
AAAAAGG569800.08.24301278-79
GCGTCGT232900.08.20012994-95
CGGGGTT112700.08.1801321
CTCGGAT90850.07.8460091
CGGCGAT61200.07.8424621
CGGGAAT73350.07.83912751
CGCGAAT11201.7144885E-77.63724951
TAGGGGG158750.07.61462250-51
CGAGAAT71200.07.5419031
CGGGAAC104350.07.5140481
GATCGGA554750.07.497611594-95
CGGGCAT124500.07.4811641
CCGGGAT105000.07.46753261
AGATCGG619800.07.34690894-95
ATCGGAA524900.07.331157794-95
Produced by FastQC (version 0.11.5)