FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625351
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAT700.00838969327.1421954
CGGCGGT1950.00445204914.6185371
AACTATC2400.001101640913.852722
GGGATTC2950.005179418711.2709113
GGGAATC3500.001490540810.85600952
ATCCACT4003.7647327E-410.6872396
CTCCAAT4901.9091269E-510.6655651
GAATCTC3650.002112665410.4107064
CCCCCCT4205.8907445E-410.1807661
ATCTTGT4701.474431E-410.1061356
CTAAAAC4256.5681693E-410.0609921
GGGGAAT3800.00294537710.0021561
TCGGAAG9300.09.70057294-95
TTTTAGC3950.00406525429.6200185
GGGATCC3950.00406525429.6200183
GAAACAT4450.00100154199.6088131
TGGGATC4450.00100448139.6057392
CTGGATT5659.125265E-59.2497811
CTGGGAT7754.2245483E-79.1955311
CAATCCA4150.00610709759.1564034