FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37170816
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG470250.010.94283480-81
TAGGGGG173750.09.74563250-51
AAAAAGG655050.09.17834678-79
CCGGGAT114450.09.0933881
CGGGAAT85600.09.0492211
CGCGAAA16850.09.0250051
AGGGGGG693800.08.8306582-83
CGGGGAT111350.08.6636961
AAGGGGG478900.08.504065582-83
TAAAAAA1086900.08.2924570-71
CGGGCAT131250.08.2915091
CGCGGAT50750.08.0530391
AAAAGGG500800.07.999272380-81
GTCGCAT57900.07.7972681
CGCGAAT11704.350659E-87.71729561
CGCAATC32750.07.6868952
CGGGGTT123350.07.62822441
CGGGATT82600.07.5943621
CTCGGAT99800.07.38071731
CGCGAGT34350.07.33240651
Produced by FastQC (version 0.11.5)