FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32525704
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG384900.09.68596580-81
CGGGGAT98200.09.4844361
CGCGTAT7402.244924E-68.9900731
GGCGAAT49600.08.8140021
CGGGGTT112200.08.0468831
AAAAAGG538800.07.97231478-79
CGGGAAT77500.07.9096071
CGGGATT63750.07.82665251
CGACCTA18000.07.65157134
AAGGGGG389050.07.63585682-83
CGGGCAT123900.07.63218161
CGAGAAT68600.07.61965851
AGGGGGG571950.07.606296582-83
CGCAAAT42500.07.6030341
GCGTCGT201300.07.575540594-95
CTCGGAT96550.07.43175841
CGCGAGT33950.07.4182771
CCGGGAT104100.07.39488121
CGCGGAT46950.07.3884651
AAAAGGG393150.07.3808180-81
Produced by FastQC (version 0.11.5)