FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32892802
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG383300.010.24077780-81
CGCGTAT7151.4076322E-69.3045971
CGCGAAT11909.094947E-129.1845211
AGGGGGG527600.08.82200682-83
CGGGAAT74850.08.7611861
CGGGGTT108000.08.6679841
AAAAAGG541900.08.65409178-79
TAGGGGG151600.08.36537150-51
CGGTAAT39000.08.2855221
AAGGGGG370450.08.23097982-83
TAAAAAA909500.07.865798570-71
CGGGGAT101550.07.8614921
CGGGAAC106650.07.842011
CCGGGAT107800.07.8024341
AAAAGGG389350.07.61155480-81
GCGGTAT37100.07.55707451
CGAATCG28650.07.45946843
GATCGGA465200.07.394054494-95
CTCGGAT94100.07.37290861
CGGCGAT65250.07.3555721
Produced by FastQC (version 0.11.5)