FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33658306
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG405950.010.815880-81
AAAAGGG424750.010.47064578-79
AAAGGGG418150.09.54050580-81
CGGGGTT108400.09.118511
AGGGGGG635500.08.94951982-83
AAAAAAG863200.08.46138476-77
CGCGAAT12009.276846E-108.31625651
CCGGGAT105700.08.1825051
TAGGGGG154600.08.01861150-51
CGGGTTT75700.07.97255851
CGCGAGT31700.07.94522951
AAAAAGG570100.07.9260578-79
CGGGAAT78700.07.60826541
GATCGGA556350.07.591382594-95
CGGGGAT107950.07.5717391
CGGGCAT121100.07.534371
ATCGGAA527600.07.47828694-95
CGCGGAT46750.07.42046451
TAAAAAA956600.07.40160670-71
AGATCGG623900.07.329138894-95
Produced by FastQC (version 0.11.5)