FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36683698
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG448500.010.32454380-81
TAGGGGG169400.010.07996250-51
CGCGATT12350.09.61953451
AGGGGGG600800.09.05535382-83
AAAAAGG620300.08.81193878-79
CGGGAAT85450.08.7867041
CGGGGTT117500.08.4930251
CCGGGAT114650.08.3311121
CGGGGAT113650.08.3207911
AAGGGGG425900.08.22421882-83
CGCGAAT14457.2759576E-128.22153951
CGCGGAT52100.08.0264951
CGGCAAT67850.07.9142471
AAAAGGG449350.07.768394580-81
CGGGCAT129800.07.7614371
CGCGAGT36500.07.68139551
TAAAAAA1048050.07.67755370-71
CCCGGAT114300.07.48354341
GATCGGA600750.07.370592694-95
GCGGTAT41600.07.3108461
Produced by FastQC (version 0.11.5)