FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28122012
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG124050.09.24657750-51
AAAGGGG326200.09.12133480-81
CCGGGAT86650.08.8841861
CGGGGAT86950.08.6349281
CGGGGTT93600.08.3260541
CGGGAAT61750.08.1571811
AAAAAGG449500.07.913228578-79
CGAGAAT59750.07.8735121
GCGTCGT209850.07.86677894-95
AGGGGGG447000.07.83599182-83
CGGGTAT35450.07.77468821
GGCGAAT42250.07.76056861
AAGGGGG319850.07.6768682-83
CTCGGAT78700.07.6683191
GCGGTAT29800.07.49467471
AAAAGGG330500.07.42917180-81
GATCGGA499500.07.41364394-95
CGCGGAT39800.07.4025241
CGTCGTG208600.07.401505594-95
ATCGGAA469650.07.318384694-95
Produced by FastQC (version 0.11.5)