FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33757655
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG395350.09.4303180-81
CCGGGAT105250.08.6689781
TAGGGGG158350.08.53345250-51
CGGGGAT96150.08.3032631
GCGTCGT278550.08.27180994-95
CTCGGAT95200.08.2363681
CGGGAAT73650.08.1299261
AAGGGGG395450.08.02898182-83
AAAAAGG562150.07.987668578-79
CGGGGTT110400.07.9202251
CGGGTAT39700.07.90027431
TAATCGA29700.07.8356234
AAAAGGG409900.07.80944380-81
CGTCGTG267650.07.73893594-95
ATCGGAA626700.07.675334594-95
AGCGTCG325700.07.584859494-95
TAAAAAA922500.07.566994770-71
CGGCAAC62500.07.5273821
GATCGGA670100.07.52207694-95
CGAGAAT69850.07.48367741
Produced by FastQC (version 0.11.5)