FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46564266
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG548900.010.13239482-83
AAAGGGG567450.010.03942780-81
CGGGGAT140450.09.5411541
AAAAGGG590250.09.52690880-81
TAGGGGG214350.09.36212250-51
AAAAAGG797150.08.58180678-79
CGGGAAT98100.08.4770081
AGGGGGG752500.08.36922882-83
CCGGGAT145450.08.3637261
TAAAAAA1341100.08.25558470-71
CGCGAAT18000.08.1839461
GGGGGGT718750.08.07651714-15
GGGGGGA776600.08.0244792
TATAGGG115450.07.7757648-49
GCGTCGT362700.07.76752594-95
CGGGCAT183100.07.75990531
GATCGGA875850.07.610330694-95
CGGGGTT154250.07.5785041
GTCGGAT53300.07.57818561
CTCGGTT96050.07.5200331
Produced by FastQC (version 0.11.5)