FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n02_HBLm29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n02_HBLm29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32872654
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGT29650.010.0972871
CGCGTAT6604.7176945E-710.0802881
TAGGGGG148350.09.01259950-51
AAAGGGG375300.08.90899880-81
CGGGGAT92900.08.7983571
CGGGAAT72800.08.7470641
CGGGGTT111300.08.5393281
GTCGCAT35900.08.2070321
GCGTCGT265250.08.02443294-95
CTCGGAT92950.07.97561261
CCGGGAT102800.07.9510441
AAGGGGG361350.07.81408982-83
CGTCGTG258150.07.79422594-95
AGGGGGG512500.07.631752582-83
CGCGGAT51300.07.59600641
GGCGAAT47950.07.5320611
AAAAAGG544550.07.52185678-79
CGCGAAT12057.318886E-87.4929951
AGCGTCG307100.07.44917594-95
ATCGGAA593600.07.39553894-95
Produced by FastQC (version 0.11.5)