FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23278426
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20381068.755342822577438No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT276380.11872795866868317TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC317550.045.912768
ATCGGAA329150.045.6165962
TCGGAAG326250.045.5991063
CGGAAGA329350.045.3847964
GATCGGA324550.044.8030361
TCTCGTA106750.034.84785542-43
GAGCACA430900.034.1439869
ATGCCGT122850.032.99790648-49
CTCGTAT101600.032.67167744-45
AAGAGCA473050.031.7433577
CGTCTGA229750.030.81406416-17
GAAGAGC488500.030.5693826
ACGTCTG232650.030.55136114-15
ACACGTC234850.030.4672412-13
CACGTCT235700.030.0956114-15
CACACGT244600.029.3498112-13
ATCTCGT113650.028.42638642-43
GCACACG259250.027.75332610-11
GGAAGAG551150.027.5080415
GTCACGA56150.026.16589228-29
Produced by FastQC (version 0.11.5)