FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27736970
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2823531.0179662738936517TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG344150.044.00499344-45
CGTATGC349100.043.67397746-47
TATGCCG357400.042.7988648-49
CTCGTAT333500.042.70429244-45
TCTCGTA349200.040.7043142-43
GAGCACA791900.040.160889
AATCTCG360150.039.31230540-41
AGAGCAC811850.039.2149358
ATGCCGT398550.038.2607548-49
ATCTCGT371450.038.15099742-43
GTATGCC404900.037.89562646-47
CGGAAGA852100.037.2041664
CCGTCTT405150.037.16898352-53
GCCGTCT406000.036.92119650-51
ATCGGAA861300.036.6196822
TCGGAAG866350.036.526633
TGCCGTC419650.036.19554550-51
GATCGGA891550.035.1790731
AAGAGCA928250.034.169537
CGTCTTC451350.033.12764752-53
Produced by FastQC (version 0.11.5)