FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34233346
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2668010.7793599842679707TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG334200.043.4743344-45
CGTATGC339250.042.99600246-47
TATGCCG350600.041.71867448-49
CTCGTAT326950.041.63463244-45
TCTCGTA350150.038.9320142-43
AATCTCG362100.037.4411240-41
GAGCACA818350.037.4115649
AGAGCAC835600.036.6277738
ATCTCGT373550.036.39784242-43
GTATGCC406100.036.19888746-47
ATCGGAA846650.035.711572
ATGCCGT412100.035.49852448-49
GCCGTCT407600.035.40661250-51
CGGAAGA858550.035.404424
TCGGAAG861400.035.259923
CCGTCTT412900.034.90664352-53
GATCGGA868550.034.6518861
TGCCGTC427500.034.04171450-51
AAGAGCA985900.031.0678337
CGTCTTC481100.029.9237852-53
Produced by FastQC (version 0.11.5)