FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences625351
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT45140.7218346176787117TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG5500.044.90515544-45
CGTATGC6150.041.70366746-47
CTCGTAT5450.041.39546244-45
AGAGCAC13900.040.320218
TATGCCG6550.039.51944448-49
GAGCACA14350.038.7248349
ATGCCGT6800.038.0665248-49
CGGAAGA15100.037.1159554
TCTCGTA6050.036.89759442-43
TGCCGTC7400.035.942850-51
GCCGTCT7300.035.1339150-51
AATCTCG6100.035.03791440-41
ATCGGAA15600.035.0129622
CCGTCTT7350.034.89490552-53
TCGGAAG15700.034.7899483
ATCTCGT6550.034.4435542-43
GATCGGA16200.033.431021
GTATGCC7950.032.56004346-47
GAAGAGC17800.031.7528386
AAGAGCA17700.031.6638977
Produced by FastQC (version 0.11.5)