FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37170816
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3496960.9407810686749519TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG427700.044.20364844-45
CGTATGC436750.043.30344446-47
CTCGTAT422050.042.40961544-45
GAGCACA934050.042.2853289
TATGCCG448050.042.15281748-49
CGGAAGA940950.041.8471834
TCGGAAG941350.041.8397183
ATCGGAA948000.041.3910262
AGAGCAC954800.041.3613978
GATCGGA949200.041.175711
TCTCGTA445450.040.0830442-43
AATCTCG453250.039.38181740-41
ATCTCGT473250.037.65818442-43
ATGCCGT502500.037.6513748-49
GTATGCC505350.037.56606746-47
CCGTCTT502850.037.13891652-53
AAGAGCA1080700.036.5603837
GCCGTCT510450.036.51581650-51
TGCCGTC529150.035.57985750-51
GAAGAGC1177350.033.658446
Produced by FastQC (version 0.11.5)