FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32525704
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2602250.8000595467510865TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG316300.043.60706344-45
CGTATGC323400.042.724646-47
TATGCCG332400.041.6032948-49
CTCGTAT312750.041.45197344-45
TCTCGTA335450.038.64058742-43
GAGCACA751450.038.537339
ATCGGAA746850.038.2446862
AGAGCAC759600.037.9800038
AATCTCG344900.037.53055240-41
TCGGAAG765950.037.4707643
GATCGGA766150.037.1802021
CGGAAGA775200.037.0539974
ATCTCGT359450.035.99452642-43
ATGCCGT389800.035.5683748-49
GTATGCC391600.035.55665646-47
CCGTCTT387200.035.11451752-53
GCCGTCT390450.034.76109750-51
TGCCGTC414100.033.3205650-51
AAGAGCA893800.032.346557
CGTCTTC444600.030.55435852-53
Produced by FastQC (version 0.11.5)