FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32892802
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT2659300.808474753838241TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG332150.043.69983344-45
CGTATGC338400.042.9425446-47
CTCGTAT327100.041.7680844-45
TATGCCG349300.041.66314748-49
ATCGGAA761300.039.282842
TCTCGTA350700.038.9590742-43
GAGCACA782500.038.6278159
TCGGAAG776750.038.5684473
AGAGCAC787150.038.2729348
GATCGGA778000.038.269411
CGGAAGA786450.038.0805554
AATCTCG362650.037.62715540-41
ATCTCGT376300.036.1697842-43
GTATGCC405250.036.08139446-47
ATGCCGT404450.035.99378648-49
CCGTCTT407650.035.2802952-53
GCCGTCT414150.034.7148750-51
TGCCGTC432400.033.63407550-51
AAGAGCA905850.033.2525187
CGTCTTC464900.031.07871652-53
Produced by FastQC (version 0.11.5)