FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33658306
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3290380.9775833638211026TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG405850.044.0614944-45
CGTATGC414350.043.4263646-47
TATGCCG422000.042.62749548-49
CTCGTAT395200.042.4726544-45
GAGCACA893650.042.1346559
AGAGCAC907300.041.4796268
CGGAAGA928150.040.4031144
ATCGGAA928850.040.3066752
TCGGAAG933150.040.1868633
TCTCGTA419850.039.9201342-43
GATCGGA950850.039.230681
AATCTCG433500.038.59117540-41
ATGCCGT477550.037.6092848-49
GTATGCC480900.037.59943446-47
ATCTCGT446500.037.4948842-43
GCCGTCT479200.036.96938750-51
CCGTCTT486850.036.58865752-53
AAGAGCA1040050.036.1897627
TGCCGTC497450.036.06654450-51
GAAGAGC1126500.033.5546886
Produced by FastQC (version 0.11.5)