FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36683698
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT3056180.8331166612482744TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG377800.043.64313544-45
CGTATGC384600.043.06236646-47
TATGCCG395050.041.8087248-49
CTCGTAT373200.041.67389344-45
TCTCGTA396800.039.1376342-43
GAGCACA909800.038.077459
AGAGCAC913000.037.876278
AATCTCG413750.037.49301540-41
ATCGGAA935450.036.6359672
ATGCCGT452900.036.47887848-49
TCGGAAG943650.036.3979383
CGGAAGA946850.036.339944
ATCTCGT429150.036.13202742-43
GTATGCC461650.036.0449346-47
GCCGTCT456000.035.6632450-51
GATCGGA958550.035.600251
CCGTCTT458950.035.52232452-53
TGCCGTC480650.034.18006550-51
AAGAGCA1067000.032.5297137
CGTCTTC524900.031.19037852-53
Produced by FastQC (version 0.11.5)