FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28122012
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2120270.7539538778377592TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG274650.043.5177144-45
CGTATGC279200.042.88831746-47
TATGCCG286050.041.68665748-49
CTCGTAT270550.041.6843844-45
TCTCGTA278150.039.2819742-43
GTCACGA313500.038.56148528-29
TCACGAG331350.036.55304330-31
ATGCCGT327700.036.41735548-49
GTATGCC331500.036.2365346-47
CGAGATT333500.036.15227532-33
ATCTCGT301750.035.9735642-43
AGTCACG340750.035.4916428-29
CCGTCTT333100.035.42088352-53
GAGCACA696150.035.3701679
GCCGTCT335200.035.17026550-51
CACGAGA348200.034.72961830-31
ACGAGAT347550.034.7181332-33
AGAGCAC710850.034.5116778
TGCCGTC350950.033.8083950-51
ATCGGAA726550.033.403692
Produced by FastQC (version 0.11.5)