FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33757655
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT2631610.7795594806570539TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG336800.043.4707144-45
CGTATGC343400.042.83561746-47
TATGCCG348050.042.03115548-49
CTCGTAT330800.041.638844-45
TCTCGTA339700.039.2353642-43
GTCACGA388700.038.45654728-29
CGAGATT408850.036.5249432-33
ATCTCGT363450.036.5146342-43
TCACGAG415700.036.1412330-31
ATGCCGT404300.036.1070448-49
AGTCACG419900.035.6839228-29
GTATGCC411200.035.67457646-47
CCGTCTT409100.035.5212252-53
GCCGTCT413500.035.11985850-51
GAGCACA877250.034.6075489
CACGAGA435850.034.31234730-31
ACGAGAT437300.034.26275332-33
TGCCGTC429250.034.0303850-51
AGAGCAC890400.033.9897238
CGGAAGA937950.032.132944
Produced by FastQC (version 0.11.5)