FastQCFastQC Report
Sat 9 Feb 2019
HTN3WBGX9_n01_HBLm30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTN3WBGX9_n01_HBLm30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46564266
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT4108400.8823074758657207TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG521550.043.87624444-45
CGTATGC531000.043.32158346-47
TATGCCG538850.042.64179248-49
CTCGTAT508500.042.59243444-45
TCTCGTA531500.039.65891642-43
GTCACGA594900.039.20556628-29
GAGCACA1235600.038.3834849
CGAGATT626150.037.3610132-33
AGAGCAC1268150.037.270928
ATGCCGT620150.037.05922748-49
TCACGAG632700.037.0405930-31
GTATGCC623500.036.9021846-47
ATCTCGT570300.036.89827342-43
AGTCACG636400.036.76088728-29
CCGTCTT628900.036.29466652-53
GCCGTCT635500.035.87242550-51
CACGAGA652400.035.81289330-31
ATCGGAA1318100.035.5453342
TCGGAAG1324900.035.4165573
ACGAGAT662900.035.37219632-33
Produced by FastQC (version 0.11.5)